Radiation - inducedReduction of Osteoblast in C 2 C 12 cells Differentiation Tomonori SAKURAI , Yohei SAWADA , Miwa YOSHIMOTO , Miyuki KAWAI and Juaji MIYAKOSHI
نویسندگان
چکیده
Bone morphogenic protein 2 (BMP-2)/HeparinlAlkali phosphatase actiyitylReal-time PCR. Therapeutic radiation causes bone damage and may increase fracture risks in treatment fbr head-andneck cancer and in pelvic irradiation. [[1iese properties can also be used fbr prevention of heterotopic ossification in hip arthroplasty, To evaluate the effects of ionizing radiation on osteoblast differentiation, C2C12 cells were directed into an osteogenic lineage by treatment with a combination of bone morphogenic protein 2 (BMP-2) (100 nglml) and heparin (30 pgtml) 6 h after irradiation (2 and 4 Gy). Osteoblast differentiation was evaluated based on alkali phosphatase (ALP) activity and expression of mRNA encod-
منابع مشابه
Ionising irradiation-induced inhibition of differentiation of C3H10T1/2 cells to the osteoblastic lineage.
PURPOSE Previous studies using mouse osteoblast derived MC3T3-E1 and mouse myoblast derived C2C12 cells have not completely explained the mechanisms responsible for osteoradionecrosis. Thus, the aim of this study was to advance the in vitro experimental approaches for investigations of osteoradionecrosis. MATERIALS AND METHODS The pluripotent stem cell line, mouse embryo derived C3H10T1/2, wa...
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تاریخ انتشار 2017